Translational Technologies

Creating Human Organs on a Chip

The Sequentially-Layered, Self-Assembly Liver Model

 

Human biomimetic organs-on-a-chip such as the liver model depicted above require the combination of: a biologically relevant matrix material; a mixture of the cell types required for organ function; media formulations to provide nutritional and hormonal needs; media flow to provide flow stimulation, drug exposure, sample oxygenation, replenishment of nutrients, and removal of waste products to recapitulate the cellular microenvironment. Platforms that combine these elements are often referred to as microphysiological systems. These components must be assembled in a chip that is robust, reproducible, and cost- and time-effective. A combination of real-time mechanistic readouts, such as high content imaging of sentinel biosensor cells, with biochemical and metabolic indicators yields a rich feature set for characterizing toxic agents and disease processes.

The interpretation of such complex data sets further requires reference data in the form of a database, such as the MPS database

 

Lee-Montiel, F.T., et al. ExpBiol Med (Maywood), 2017