Positions Available

Research Technician II

The Research Technician II works within the matrix environment of the Drug Discovery Institute (DDI) assisting investigators and collaborators in the development, optimization and implementation of assays in the area of liver Microphysiology systems for multiple disease models. Assays will be biochemical and cell based assays with emphasis on phenotypic and high-content assays.

A B.S. in biochemistry, microbiology, cell biology, chemistry or biology and 1-2 years’ experience are required. This position will require experience in biochemistry, molecular biology, cell culture and fluorescence microscopy. Experience in tissue culture, microfluidics, and HTS detection instruments is desired.

Qualified candidates should send resume and cover letter to cmg72@pitt.edu

Research Technician III

The Research Technician III works within the matrix environment of the Drug Discovery Institute (DDI) assisting investigators and collaborators in the development, optimization and implementation assays for the identification and characterization of biological probes and/or drug lead candidates. Assays will be cell based assays with emphasis on phenotypic and high-content assays.

A B.S. or Masters and 2-3 years’ experience are required. This position will require experience in biochemistry, molecular biology, cell culture, and fluorescence microscopy. Experience in mammalian cell culture and assay development, validation and/troubleshooting is a required. Candidate must have hands on experience in immunohistochemistry of fluorescent probes for platforms such as flow cytometry, High Content Analysis or fluorescence microscopy. Experience in assay automation and using automated/robotic liquid handling and HTS detection instruments is desired.

Qualified candidates should send resume and cover letter to cmg72@pitt.edu

Postdoctoral Associate – Computational Biology

The Lezon lab at the University of Pittsburgh Drug Discovery Institute (UPDDI) seeks a full-time postdoctoral associate to identify novel combination therapeutics for treating Alzheimer’s disease (AD). The incumbent will be responsible for developing methods that integrate existing information on drug structures, targets and activities with results from ongoing cell-based screens to generate therapeutic strategies for AD. The successful applicant will be expected to lead the development of new computational drug discovery strategies, and to work with a multi-disciplinary team to design experiments that test computational predictions.

UPDDI is a world-class drug discovery institute focused on applying quantitative systems pharmacology (QSP) to develop cures for complex diseases. Our Alzheimer’s disease program is a collaboration between pharmacologists, neurobiologists and computational biologists that combines phenotypic screens with computational inference and modeling to better understand and treat AD. We have our own state-of-the-art experimental facilities and full access to the computational resources of the Department of Computational and Systems Biology.

Qualifications: Candidates must have strong quantitative and programming skills, knowledge of network inference, a demonstrated ability to find creative solutions to novel problems, and the ability to communicate effectively with a transdisciplinary team. Knowledge of systems biology and machine learning a strong plus.

Contact: Qualified candidates should email a CV, a brief description of scientific accomplishments, and the names of three references to lezon@pitt.edu.

Computational Biologist – Transcriptomic Network Inference

The University of Pittsburgh Drug Discovery Institute (UPDDI) is part of a multi-scale multidisciplinary effort to identify biomarkers related to traumatic brain injury (TBI) and to develop a drug discovery pipeline for treating TBI-related neurodegeneration. UPDDI invites applications for a full-time postdoctoral position in Computational Systems Biology. The incumbent will be responsible for developing a quantitative systems pharmacology (QSP) pipeline for using transcriptomic data to predict drugs that reduce TBI-associated neurodegeneration. The primary duties are analysis of RNA sequencing data from in vitro TBI models; inferring signaling pathways and specific targets related to TBI progression and recovery; and network- or structure-based computational prediction of drugs that modulate the course of TBI by targeting these pathways.

Qualifications: The ideal candidate will have a PhD in computational biology, bioinformatics, or a related field, with research focus on causal inference from transcriptomic data. Candidates are expected to be familiar with RNASeq data and related analysis tools and pipelines. Proficiency in a scripting language (e.g., Python, R, Perl) is required; advanced coding skills are highly desired. Familiarity with pathway analysis software, and experience with drug discovery or QSP is a plus.

Post-Doctoral Position in Human Liver Microphysiology Systems (MPS)

In the laboratory of D. Lansing Taylor, Ph.D., Director, University of Pittsburgh Drug Discovery Institute (UPDDI) Distinguished Professor and Allegheny Foundation Professor of Computational and Systems Biology

Start Date: Fall 2018

Funding from NIH-NCATS, the EPA, and NIH-NIDDK, NIH-CTSA

Experience and Training

• Ph.D in physiology, pathology, cell biology, biophysics, biomedical engineering, or related field

• Experience in liver cell and organ physiology, cell biology and molecular biology

• Experience in using automated imaging systems preferred

• Experience with primary human cells and iPSCs a plus.

Position Responsibilities

• Characterize the structure and functions of a human, 4 cell-type, liver disease MPS including non-alcoholic fatty liver disease and/or type 2 diabetes over one month based on primary human cells and iPSC-derived cells

• Collaborate with colleagues to incorporate fluorescence-based biosensors into one or more cell types

• Collaborate with colleagues to integrate the human liver MPS with other organ MPS including pancreatic islets, white adipose tissue, intestine

• Plan and execute experiments, analyze and present results in presentations and publications

• Collaborate with the liver microphysiology systems team and clinician-scientists at Pitt

Job Description

UPDDI is searching for a Postdoctoral Fellow to apply a human liver Microphysiology System (MPS) for the development and analysis of human, 3D cellular models of liver diseases using both primary human cells and iPSC-derived cells. Expertise in the liver, use of fluorescence imaging microscopy of cellular systems is required. Experience with 3D biological models preferred. This is an excellent opportunity to get involved in a series of high profile collaborations in developing and applying microfluidic organ models for disease and toxicology testing.

Post-Doctoral Biomedical Engineer Position in Human Liver Microphysiology Systems (MPS)

In the laboratory of D. Lansing Taylor, Ph.D., Director, University of Pittsburgh Drug Discovery Institute (UPDDI), Distinguished Professor and Allegheny Foundation Professor of Computational and Systems Biology /p>

Start Date: Fall 2018

Funding from NIH-NCATS, the EPA, and NIH-NIDDK, NIH-CTSA

Experience and Training

• Ph.D in biophysics, biomedical engineering, or related field

• Experience in microfluidics

• Experience in cell biology/cell cultures

• Experience in high content imaging or confocal microscopy

• Experience with iPSCs a plus.

Position Responsibilities

• Extend the development of a human, 4 cell-type, microphysiology liver model using an all glass microfluidic device and oxygen sensing techniques to construct a vascularized, zonated liver acinus.

• Characterize the structure and functions of a liver disease model MPS for over a month (options include hepatocarcinoma, metastatic breast or melanoma cancers, non-alcoholic fatty liver disease and Type 2 Diabetes) using a variety of readouts including imaging fluorescence-based biosensors, biochemistry and mass spectrometry.

• Plan and execute experiments, analyze and present results in presentations and publications

• Collaborate with the liver microphysiology systems team and clinician-scientists at Pitt

Job Description

UPDDI is searching for a Postdoctoral Microphysiology Biomedical Engineer for the development and analysis of human, 3D cellular models of organs and disease based on microfluidic devices. Expertise in microfluidics and experimental use of fluorescence imaging microscopy of cellular systems is required. Experience with 3D biological models preferred. This is an excellent opportunity to get involved in a series of high profile collaborations in engineering microfluidic organ models for disease modeling and toxicology testing.

Post-Doctoral Position in Molecular and Cellular Biology Designing, Constructing and Applying Fluorescence-based Cellular Biosensors for Human Microphysiology Systems of the Liver and Pancreatic Islets.

In the laboratory of D. Lansing Taylor, Ph.D., Director, University of Pittsburgh Drug Discovery Institute (UPDDI) Distinguished Professor and Allegheny Foundation Professor of Computational and Systems Biology

Start Date: Fall 2018

Funding from NIH-NCATS, the EPA, and NIH-NIDDK, NIH-CTSA

Experience and Training

• Ph.D in molecular biology, biochemistry, cell biology or related field

• Experience in designing and constructing fluorescence-based biosensors using fluorescent proteins

•Experience with lentiviral transduction methods for delivering biosensor constructs to cells

•Experience with CRISPR/Cas 9 a plus

• Experience in using automated imaging systems a plus

• Experience with primary human cells and iPSCs a plus

Position Responsibilities

• Design, construct and apply fluorescence-based biosensors to report on pathophysiology in a human, 4 cell-type, liver disease MPS including non-alcoholic fatty liver disease and type 2 diabetes over one month based on primary human cells and iPSC-derived cells

•Collaborate with colleagues to integrate the human liver MPS with other organ MPS including pancreatic islets, white adipose tissue, intestine

• Plan and execute experiments, analyze and present results in presentations and publications

• Collaborate with the liver microphysiology systems team and clinician-scientists at Pitt

Contact: Qualified candidates should email a CV and names of two references to Christine Grabowski at cmg72@pitt.edu

Mailing Address:

Christine Grabowski

Administrator

Drug Discovery Institute

University of Pittsburgh School of Medicine

W955 Biomedical Science Tower

200 Lothrop Street

Pittsburgh, PA 15261