UPDDI Core Facilities and Equipment

Preclinical Resources

Drs Eiseman and Beumer closely collaborate on numerous preclinical projects. The combined resources available for preclinical studies are listed below.

In vitro

The equipment available for in vitro/ex vivo work includes: analytical balances, , microcentrifuges, table top centrifuges, (fluorescent) microscopes, laminar flow hoods, CO2 incubators, shaking water baths, liquid nitrogen storage for cells, refrigerators, freezers, gel electrophoresis, microplate readers, pH meters, Z1 Coulter counter, light box, and access to the shared use equipment within UPCI which includes: liquid scintillation counters, gamma counters, X-ray film developers, Molecular Dynamics Densitometer, ultracentrifuges, autoclaving and glass washing services, Tecan Safire and Licor Odyssey, etc.

In vivo

The Division of Laboratory Animal Resources University of Pittsburgh maintains the animal care facilities at the Hillman Cancer Center, Basic Research Pavilion, and is fully accredited by the American Association for Accreditation of Laboratory Animal Care. Within the Central Animal Facility, Dr Eiseman has a dedicated laboratory (G11.8) and fully equipped animal procedure room (G11.9), as well as an animal housing room (G11.28a) capable of handling 1500 rodents at any time.

Analytical

Analytical equipment available for preclinical research includes a Perkin-Elmer model AAnalyst 600 Atomic Absorption spectrometer, two regular HPLC UV systems with dual wavelength, fluorescence, one HPLC with UV and radioactivity detection, four Waters QuattroMicro triple stage mass spectrometers with UV, and two Applied Biosystems 4000Q hybrid triple-quadrupole, linear ion-trap with a diode array detector. All mass spectrometric detectors are supplied with high purity nitrogen generated by a Parker Balston nitrogen generator model 75-880, supplied with compressed air by a Copto air-compressor located in the basement of UPCI.